Bovine Heat Shock 70kDa Protein 1B (HSPA1B) ELISA Kit

The Bovine Heat Shock 70kDa Protein 1B (HSPA1B) ELISA Kit from Assay Genie quantifies HSP70-2, the inducible 70 kDa ATP-dependent chaperone encoded by the HSPA1B gene on bovine chromosome 23. HSP70-2 binds misfolded polypeptides and supports refolding during thermal, oxidative, and inflammatory stress, making it a leading biomarker for cellular stress responses in cattle.

Veterinary physiologists and animal scientists apply this assay to measure HSP70-2 concentrations in tissue homogenates and fluid specimens, supporting investigations of heat-stress resilience in dairy herds, transport-related welfare, postpartum recovery, vaccine-induced immune activation, and metabolic challenge in feedlot cattle. The kit supplies precoated wells, biotinylated detection antibody, streptavidin-HRP conjugate, TMB substrate, recombinant standards, and wash buffers in a complete workflow.

Standard handling requires refrigeration at 4 degrees C, with reagents remaining stable through the stated shelf life when aliquoted to avoid repeated freeze-thaw cycles. The kit is intended for research use only and supports longitudinal study designs and herd-level sampling protocols in bovine stress biology and veterinary immunology research.