Bovine Dopamine beta-hydroxylase (DBH) ELISA Kit

The Bovine Dopamine beta-hydroxylase (DBH) Sandwich ELISA Kit from Assay Genie quantifies DBH, the copper-containing enzyme that converts dopamine to norepinephrine in catecholamine biosynthesis. The 96-well microplate is pre-coated with a capture antibody, paired with biotinylated detection antibody and streptavidin-HRP for colorimetric readout measured at 450 nm.

Serum and plasma samples are validated for accurate measurement, and the kit is calibrated against purified bovine DBH standards covering a working detection range of 0.5 to 50 ng/mL depending on standard curve preparation. Investigators studying bovine stress physiology, cardiovascular regulation, sympathetic nervous system activity, or adrenal medulla function can apply this assay to plasma samples collected under defined experimental protocols.

Storage guidance: unopened kits should be stored at 2-8 °C; reconstituted reagents and standards require frozen storage at -20 °C. Allow all components to equilibrate to room temperature before use, and limit repeated freeze-thaw cycles to preserve antigen integrity. Each pack provides reagents for 96 wells, including pre-coated plate, standards, detection antibody, streptavidin-HRP, substrate, wash buffer, and stop solution, supporting in vitro research applications in catecholamine pathway studies.