Bovine Defensin Beta 1 (DEFb1) ELISA Kit

Beta-defensin 1 (DEFb1) is a small cationic antimicrobial peptide secreted by epithelial tissues of cattle, where it contributes to innate mucosal defense. Quantifying this peptide in blood and tissue samples supports bovine research into mastitis susceptibility, reproductive immunology, gut barrier function, and respiratory disease resistance.

This colorimetric immunoassay from Assay Genie is calibrated specifically against the bovine DEFb1 sequence, providing species-specific quantification for cattle-derived samples. The plate-based format includes pre-coated wells, a recombinant standard, detection antibody, and substrate reagents to run a complete standard curve alongside unknown samples in a single batch.

Acceptable sample matrices include serum or plasma fractions, as well as tissue homogenates. The kit is supplied for in vitro research use only. Reagents should be kept refrigerated between uses, protected from light, and brought to room temperature before each run to maintain reproducible binding kinetics.