Bovine cTn-I (Cardiac Troponin-I) ELISA Kit (BOFI00110)

Cardiac troponin I (cTn-I) is a 24 kDa regulatory subunit of the thin filament that controls calcium-dependent actin-myosin interaction in cardiomyocytes. Release of cTn-I into the bloodstream follows myocardial cell injury, establishing it as a sensitive biomarker for cardiac damage in cattle. This Assay Genie kit enables quantitative measurement of bovine cTn-I in research specimens, supporting veterinary cardiology studies of traumatic reticulopericarditis, endotoxemia, parturient complications, and stress-related cardiomyopathy in dairy and beef herds.

The kit is supplied as a complete reagent set configured for 96 Assays, including a pre-coated microplate, lyophilized protein standards, biotinylated detection antibody, streptavidin-HRP conjugate, concentrated wash buffer, TMB substrate, and stop solution. A standard curve generated on each plate allows interpolation of unknown analyte concentrations. The protocol follows a multi-step immunoassay workflow with incubation and washing cycles applied to serum or plasma aliquots, or tissue homogenate, prepared from cattle under standard veterinary handling.

For research use only, this kit is intended for in vitro laboratory investigations in academic, veterinary, and animal health research settings. Reagents should be stored refrigerated upon arrival to maintain stability through the printed expiration date. Researchers are advised to run specimens in duplicate with appropriate internal controls and to consult the lot-specific certificate of analysis for exact working concentrations of reconstituted standards and conjugate.