Bovine CD86 (cluster of differentiation 86) ELISA Kit (BOFI00138)

Quantify cluster of differentiation 86 (CD86) in cattle with this enzyme-linked immunosorbent assay from Assay Genie. The assay targets soluble CD86, the B7-2 co-stimulatory molecule expressed on activated antigen-presenting cells, and produces a quantitative readout from a recombinant protein standard curve. Investigators studying bovine immune activation, vaccine efficacy, and infectious disease pathogenesis in dairy and beef herds can use the kit to monitor co-stimulatory signaling in circulating lymphocytes and tissue-derived samples.

The 96 Assays pack provides a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, lyophilized CD86 standard, dilution buffer, concentrated wash buffer, TMB substrate, and stop reagent. Reconstituted standard should be stored refrigerated in single-use aliquots to limit freeze-thaw exposure. Each component vial carries an individual expiration date on the label.

For in vitro research use only, not for diagnostic procedures. Colorimetric signal is captured on a standard microplate reader with the appropriate filter, and absorbance values are interpolated against the standard curve. The assay workflow supports batch processing of unknown samples alongside the calibrator series for reliable inter-assay comparison.