Bovine CCL4 (MIP-1 beta)DIY ELISA Kit

The Bovine CCL4 (MIP-1 beta) DIY ELISA Kit from Assay Genie is supplied as a flexible, do-it-yourself immunoassay format intended for researchers studying bovine chemokine signaling. CCL4, also called macrophage inflammatory protein-1 beta, is a CC-chemokine ligand for CCR5 and is secreted by macrophages and lymphocytes during inflammatory responses, making it a useful marker in cattle health, mastitis, and infectious disease research.

The DIY configuration provides the capture antibody, biotinylated detection antibody, recombinant bovine CCL4 standard, streptavidin-HRP, wash buffer, and substrate in quantities sufficient for up to 10 user-supplied microplates. Researchers coat their own plates, then run the immunoassay workflow with the provided reagents. Reagents should be stored according to the supplied protocol; the standard and detection antibody components are kept frozen to maintain activity, while diluted wash buffer remains stable at room temperature. Reconstituted standards should be aliquoted to limit repeated freeze-thaw cycles.

Assay Genie supplies this research-use-only kit for measuring endogenous CCL4 in bovine cell culture supernatant and serum samples from experimentally treated cattle, supporting studies of innate immunity, vaccination response, and pathogen challenge models.