Bovine CALCA (Calcitonin) ELISA Kit

The Assay Genie Bovine CALCA (Calcitonin) ELISA Kit is a quantitative Sandwich immunoassay built on a pre-coated 96-well microplate, enabling researchers to measure calcitonin concentrations in bovine serum and plasma with a colorimetric readout. Calcitonin is a 32-amino-acid peptide hormone secreted by parafollicular C-cells of the thyroid gland that plays a central role in calcium homeostasis and bone metabolism, opposing the action of parathyroid hormone by inhibiting osteoclast-mediated bone resorption. This ELISA kit is intended for research use only and supports studies in endocrinology, veterinary physiology, skeletal biology, and metabolic disorders where circulating calcitonin serves as a biomarker of thyroid and skeletal function.

Reagents should be stored refrigerated at 2-8 °C; the standard, detection antibody, and streptavidin-HRP components remain stable through the stated expiration when handled according to the package insert. Reconstituted standards should be aliquoted and frozen to limit freeze-thaw cycles, and the microplate strips should be kept dry and protected from light. The assay typically requires 50 µL of sample per well, with a runtime of approximately 3-4 hours, and produces results readable on any standard microplate reader at 450 nm.