BLNK (Phospho-Tyr84)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this colorimetric cell-based ELISA kit is designed to detect phosphorylated BLNK (B-cell linker protein) at Tyr84 in cell lysates, supporting sensitive and quantitative measurement of BLNK phosphorylation in studies of B cell receptor signalling and immune cell pathways. The assay uses an indirect ELISA format, in which target-specific primary antibodies capture BLNK (Phospho-Tyr84) and HRP-conjugated secondary antibodies bind the Fc region of the primaries, catalysing a colorimetric reaction upon substrate addition. The kit is described by the manufacturer as lysate-free and high-throughput, and is suitable for measuring relative levels of phosphorylated BLNK in cultured cells, including assessment of the effects of various treatments, inhibitors, and activators.

The kit is reactive with human, mouse, and rat samples and is intended for use with adherent cells. Detection is colorimetric at 450 nm, with a stated dynamic range of > 5000 Cells. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalising target absorbance values, and a Crystal Violet whole-cell staining step is provided to determine cell density so that absorbance values can be adjusted for plating differences.

BLNK is a cytoplasmic adaptor protein that links B-cell receptor-associated kinases with downstream signalling pathways, including PLCG1 activation and Ca2+ mobilisation, and is phosphorylated on tyrosine residues by SYK and LYN following BCR activation. Altered BLNK function is relevant to immune disorders such as autoimmune disease and lymphoma, and the kit is positioned for immunology research investigating these processes.