BIM Colorimetric Cell-Based ELISA Kit

From Assay Genie, this colorimetric Cell-Based ELISA Kit is designed for the accurate detection of BIM (Bcl-2 interacting mediator of cell death) levels in cell lysates, supporting studies of BIM's role in cell death pathways. The assay has a stated dynamic range of > 5000 Cells, uses colorimetric detection at 450 nm, and is reactive with Human, Mouse, and Rat samples, with adherent cells listed as the intended sample type. The kit is described as a convenient, lysate-free, high-throughput format suitable for measuring the relative amounts of BIM in cultured cells and for screening the effects that various treatments, inhibitors (such as siRNA or chemicals), or activators have on BIM expression.

Qualitative determination of BIM concentration is achieved through an indirect ELISA format in which BIM is captured by target-specific primary antibodies, and the bound primary antibody is in turn detected by HRP-conjugated secondary antibodies that catalyze a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control for normalising the target absorbance values, and a Crystal Violet whole-cell staining step may be used to determine cell density so that absorbance values can be normalised to cell amounts and plating differences adjusted. The kit is recommended for storage at 4°C with a stated stability of 6 Months.

BIM is a pro-apoptotic member of the BCL-2 protein family (gene symbol BCL2L11; Gene ID 10018; UniProt ID O43521) that interacts with BCL2, BCL2L1/BCL-X(L), and MCL1 and acts as an apoptotic activator. Its dysregulation has been implicated in various diseases including cancer, making it a valuable biomarker for research into apoptosis, neuronal and lymphocyte cell death, thymocyte-negative selection, and potential therapeutic targets.