Bcr (Phospho-Tyr177)Colorimetric Cell-Based ELISA Kit

A sensitive cell-based ELISA from Assay Genie is designed to detect and quantify levels of BCR (Breakpoint cluster region) protein phosphorylated at tyrosine 177 in cultured cells. The lysate-free, high-throughput assay uses a 96-well microplate format and an indirect ELISA approach, in which phospho-specific primary antibodies are paired with HRP-conjugated secondary antibodies that catalyze a colorimetric reaction measured at 450 nm after substrate addition. It is reactive with human and mouse samples and is suited to adherent cells, with a dynamic range of > 5000 Cells.

The kit includes a monoclonal anti-GAPDH antibody as an internal positive control for normalizing target absorbance, and a Crystal Violet whole-cell staining step that can be used to determine cell density and adjust for plating differences. It supports screening of the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on Bcr phosphorylation. According to the manufacturer, phosphorylation of BCR at tyrosine 177 plays a role in downstream signaling related to cell proliferation and survival, and dysregulation has been implicated in diseases including leukemia and other cancers, making the kit a useful tool for studies of cellular signaling, disease mechanisms, and potential therapeutic targets.