BCAR1 (Phospho-Tyr410)Colorimetric Cell-Based ELISA Kit

A colorimetric cell-based ELISA kit from Assay Genie is designed for the accurate detection of BCAR1 phosphorylated at Tyrosine 410 in cell lysates. The assay offers high sensitivity and specificity for researchers studying cancer and cell signaling pathways. BCAR1, also known as p130Cas, is a key adaptor protein involved in cell adhesion, migration, and proliferation; phosphorylation at Tyrosine 410 plays a critical role in signaling cascades related to cell growth and survival.

This lysate-free, high-throughput kit uses an indirect ELISA format in which HRP-conjugated secondary antibodies catalyze a colorimetric reaction measured at 450 nm. The dynamic range is > 5000 Cells, and the kit is reactive with human, mouse, and rat samples. An anti-GAPDH monoclonal antibody is included as an internal positive control, and Crystal Violet whole-cell staining can be used to normalize absorbance values to cell density. The format supports measuring relative phosphorylated BCAR1 levels and screening the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on BCAR1 phosphorylation in adherent cells.

Dysregulation of BCAR1 phosphorylation has been associated with cancer and autoimmune disorders, making it a target of interest for therapeutic research. Related database references include Gene ID 9564 and UniProt ID P56945. The kit provides a reliable tool for studying BCAR1 signaling pathways and developing potential therapeutic strategies.