BCAR1 (Phospho-Tyr249)Colorimetric Cell-Based ELISA Kit

A cell-based ELISA kit from Assay Genie designed for the detection of phosphorylated BCAR1 at tyrosine 249. The assay uses a colorimetric detection method read at 450 nm and follows an indirect ELISA format, in which BCAR1 (Phospho-Tyr249) is captured by specific primary antibodies and detected using HRP-conjugated secondary antibodies that catalyze a colorimetric reaction upon substrate addition.

The kit is validated for use with adherent cell samples and is reactive with human, mouse, and rat species. It can be used both to measure relative levels of phosphorylated BCAR1 in cultured cells and to screen for the effects of treatments, siRNA, chemicals, or other modulators on BCAR1 phosphorylation. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalization, and a Crystal Violet whole-cell staining method can be used to determine cell density and adjust for plating differences. The dynamic range is reported as greater than 5000 cells.

As a qualitative, lysate-free, high-throughput assay, the kit provides a user-friendly protocol for studying BCAR1 signaling in cell biology research, including pathways related to cell adhesion, migration, and cancer-related processes such as antiestrogen resistance in breast cancer cells.