BAD (Phospho-Ser136)Colorimetric Cell-Based ELISA Kit

This cell-based ELISA kit from Assay Genie is intended for the detection of BAD protein phosphorylation at the Ser136 site in adherent cell samples. The assay uses a colorimetric detection method measured at 450 nm and is configured as a Cell-Based ELISA. The kit shows reactivity with Human, Mouse, and Rat samples and has a stated dynamic range of > 5000 Cells.

Detection relies on an indirect ELISA format in which BAD (Phospho-Ser136) is captured by target-specific primary antibodies, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody to catalyze a colorimetric reaction upon substrate addition. The workflow is described as lysate-free, high throughput, and sensitive, and is suitable for measuring relative amounts of phosphorylated BAD in cultured cells as well as for evaluating the effects of treatments, inhibitors, or activators on BAD phosphorylation.

For normalization, a monoclonal antibody specific for human GAPDH is included as an internal positive control, and Crystal Violet whole-cell staining can be used to determine cell density. Associated database entries for the BAD target include UniProt ID Q92934, Gene ID 572, OMIM 603167, and Unigene Hs.370254.