B-RAF (Phospho-Ser602)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this colorimetric cell-based ELISA kit is designed for the accurate detection of phosphorylated B-Raf at Ser602 in cell lysates. The kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications. It is a convenient, lysate-free, high-throughput format suitable for adherent cells that can detect B-RAF protein phosphorylation and expression profile in cells, with reactivity to Human, Mouse, and Rat samples and a dynamic range of > 5000 Cells.

Phosphorylated B-Raf at Ser602 is a key signaling molecule in the MAPK pathway, regulating cell growth, proliferation, and survival. Dysregulation of this pathway has been linked to various diseases, including cancer, making detection of phosphorylated B-Raf at Ser602 essential for studying disease mechanisms and potential therapeutic interventions. The kit can be used for measuring the relative amounts of phosphorylated B-RAF in cultured cells as well as screening for the effects that various treatments, inhibitors (such as siRNA or chemicals), or activators have on B-RAF phosphorylation.

Detection is achieved by an indirect ELISA format, in which B-RAF (Phospho-Ser602) is captured by specific primary antibodies and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. The HRP enzyme catalyzes a colorimetric reaction upon substrate addition, measured at 450 nm. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing target absorbance values, and a Crystal Violet whole-cell staining method may be used to determine cell density and adjust for plating differences.