B-Myb Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Colorimetric Cell-Based ELISA Kit provides a qualitative, lysate-free method for detecting B-Myb protein expression profiles in cultured cells. Using an indirect ELISA format, B-Myb-specific primary antibodies capture the target, and HRP-conjugated secondary antibodies catalyze a colorimetric reaction read at 450 nm. The assay is suitable for measuring relative B-Myb amounts in cultured cells and for screening the effects of treatments, inhibitors (such as siRNA or chemicals), or activators.

Configured for use with adherent cells, the kit has a dynamic range of > 5000 Cells and reactivity against Human, Mouse, and Rat samples. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalizing target absorbance values, and a Crystal Violet whole-cell staining step can be used after the HRP readout to determine cell density. The target, B-Myb, is encoded by the MYBL2 gene and functions as a transcription factor involved in cell cycle progression, with roles in cell proliferation and differentiation.

Positioned within the Epigenetics and Nuclear Signaling research area, the kit is intended for storage at 4°C with a stability of 6 Months. It is described as a high-throughput, sensitive assay intended for research applications.