Avidin-Alk Phosphatase Conjugate, 200 uL

The Avidin-Alkaline Phosphatase Conjugate from STEMCELL Technologies is a biotin-binding detection reagent supplied as a 200 uL liquid in a single vial. Leveraging the exceptionally high affinity of avidin for biotin (Kd approximately 10^-15 M), this reagent localizes alkaline phosphatase enzymatic activity at any biotinylated target, enabling chromogenic signal generation in ELISA, western blot, and immunohistochemistry workflows. It functions as the recommended secondary reporter for STEMCELL's STEM-ELISA human pluripotency marker kits, pairing with pNPP substrate for colorimetric readout at 405 nm or with BCIP/NBT for blot-based detection. The conjugate is typically diluted 1:1000 in assay buffer before use, and the 200 uL volume provides sufficient reagent for hundreds of standard microwell wells. Store refrigerated at 4°C, protect from light, and minimize freeze-thaw cycles to preserve enzymatic activity. For research use only.