ATF5 Transcription Factor Activity Assay

The ATF5 (Activating Transcription Factor 5) Transcription Factor Activity Assay from Assay Genie is a DNA-binding ELISA that quantifies the DNA-binding activity of ATF5 in nuclear and cell lysates from human, mouse, and rat samples. The microplate is pre-coated with a specific double-stranded oligonucleotide containing the consensus binding sequence for ATF5, allowing active transcription factor from nuclear extract to bind. A primary antibody specific for ATF5 is then added, followed by an HRP-conjugated secondary antibody and TMB substrate. Use approximately 10 µL of nuclear extract per well as the sample volume. The assay is intended for research use only and is shipped refrigerated; store kit components at 4 °C and protect from light. This kit supports studies on cellular stress responses, neural differentiation, and oncogenic signaling, as ATF5 is a basic leucine zipper transcription factor implicated in the integrated stress response, glioblastoma, and certain leukemias.