ATF3 Transcription Factor Activity Assay

ATF3 (Activating Transcription Factor 3) is a stress-responsive member of the ATF/CREB transcription factor family that binds the cAMP response element (CRE) consensus sequence to regulate genes involved in DNA damage response, endoplasmic reticulum stress, and apoptosis signaling. This Assay Genie DNA-Binding ELISA quantifies active ATF3 binding capacity in nuclear extracts, providing a functional readout that complements total protein measurements obtained by western blot.

Immobilized CRE oligonucleotides capture ATF3 from prepared nuclear and cell lysates, with detection via an ATF3-specific antibody and a colorimetric substrate read on a microplate. Each kit includes reagents for 96 assays and is intended for research use only. Store components at 4°C according to the package insert, and aliquot working reagents to avoid repeated freeze-thaw cycles. The assay supports studies of inflammatory signaling, oncogenic stress pathways, and transcriptional regulation across human, mouse, and rat model systems.