ATF2 (Phospho-Thr73)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit is designed for the detection of phosphorylated ATF2 at Threonine 73, providing a sensitive and specific tool for research into cellular stress signalling pathways and gene regulation.

The kit uses a colorimetric detection method at 450 nm in an indirect ELISA format, in which the phosphorylated target is captured by specific primary antibodies and detected using HRP-conjugated secondary antibodies that catalyse a colorimetric reaction upon substrate addition. It is described as a convenient, lysate-free, high-throughput assay that can detect ATF2 protein phosphorylation and expression profiles in cells, suitable for measuring relative amounts of phosphorylated ATF2 in cultured cells and for screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators.

Reactivity is reported for Human, Mouse and Rat samples, with a dynamic range of > 5000 Cells. The assay is performed on adherent cells and includes a monoclonal antibody specific for human GAPDH as an internal positive control for normalising target absorbance values, alongside a Crystal Violet whole-cell staining method used to determine cell density. The target is the ATF2 gene product (Gene ID: 1386; UniProt ID: P15336), a transcription factor of the leucine zipper family that binds the cAMP-responsive element (CRE).