AS160 Signaling ELISA Sampler Kit

The Assay Genie AS160 Signaling ELISA Sampler Kit supports research into insulin signaling and glucose transport by quantifying Akt Substrate of 160 kDa (AS160, also called TBC1D4), a Rab-GTPase activating protein central to GLUT4 vesicle translocation in skeletal muscle and adipose tissue. The sampler configuration includes three independent 48-well microplates, providing up to 144 reactions to profile total or phosphorylated AS160 across multiple treatment conditions, time courses, or donor cohorts. The colorimetric assay specifically detects human AS160 with cross-reactivity to mouse and rat TBC1D4 and is validated for serum or EDTA plasma specimens, requiring a 50 µL sample volume per well. The kit is intended for research use only, supporting studies in type 2 diabetes, exercise metabolism, and metabolic syndrome research. Components are shipped refrigerated and should be stored at 4 degrees C; reagents remain stable through multiple freeze-thaw cycles when aliquoted. Each 48-well plate can be run independently, allowing flexible scheduling for small pilot studies or larger screening campaigns.