ARC Colorimetric Cell-Based ELISA

From Assay Genie, this Cell-Based ELISA kit is designed for the qualitative detection of ARC (Apoptosis Repressor with CARD domain) protein expression in cultured cells. The indirect assay format uses ARC-specific primary antibodies, with HRP-conjugated secondary antibodies binding the Fc region of the primary antibody to catalyse a colorimetric reaction upon substrate addition.

Working in a 96-Well Microplate format with colorimetric detection at 450 nm, the kit is intended for use with adherent cells and reports a dynamic range of > 5000 Cells. The workflow is lysate-free and high-throughput, suitable for measuring relative ARC levels in cultured cells and for screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on ARC expression.

Because the assay is qualitative, two normalisation methods are included: a monoclonal anti-GAPDH antibody as an internal positive control for normalising target absorbance values, and Crystal Violet whole-cell staining to determine cell density and adjust for plating differences. The target, ARC, is encoded by the NOL3 gene (Gene ID: 8996; UniProt ID: O60936-2), an anti-apoptotic protein reported to down-regulate the enzyme activities of caspase 2, caspase 8 and tumor protein p53. The kit is stored at 4°C for 6 months.