APLP2 Colorimetric Cell-Based ELISA Kit

Amyloid Precursor-Like Protein 2 (APLP2) is a member of the APP (amyloid precursor protein) family and has been implicated in neuronal development, synaptic plasticity, neuromuscular transmission, and the pathogenesis of Alzheimer's disease. For researchers studying this protein, Assay Genie offers a Cell-Based ELISA Kit designed for the qualitative detection and relative quantification of APLP2 in cultured cells.

The kit uses an indirect ELISA format in which APLP2 is captured by APLP2-specific primary antibodies and detected using HRP-conjugated secondary antibodies. A colorimetric reaction is generated upon substrate addition and read at 450 nm, with a stated dynamic range of > 5000 Cells. The assay is described as lysate-free, high throughput, and sensitive, and can be used to screen the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on APLP2 expression. Reactivity is reported for human, mouse, and rat samples. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalization, and Crystal Violet whole-cell staining is recommended for determining cell density.

Storage is at 4°C with a stated stability of 6 Months. The kit is intended for adherent cell culture in a 96-well microplate format. APLP2 has a reported molecular weight of 87,215 Da and is referenced under UniProt ID Q06481 and NCBI Gene ID 334.