AP-2 Transcription Factor Activity Assay

The AP-2 (Activator Protein-2) family regulates gene expression by binding to GCC-N3-GGC motifs in promoter regions of target genes, with documented roles in neural crest development, epithelial differentiation, and oncogenic signaling pathways. This DNA-binding ELISA from Assay Genie captures active AP-2 from nuclear extracts using consensus oligonucleotide-coated wells, then quantifies bound factor through an antibody read-out measured on a standard microplate reader. Researchers commonly apply the kit to investigate transcription factor activation in cancer cell lines, stem cell differentiation models, and chromatin regulation studies.

Each kit provides reagents sufficient for 96 Assays; aliquot working solutions upon receipt to minimize freeze-thaw cycles and store the components at -20 degrees C for long-term stability. Compatible samples include nuclear and cell lysates prepared from human, mouse, or rat tissues and cultured cells, with reactivity validated across these three species. The assay is intended for research use only and pairs well with downstream western blot or ChIP analysis to confirm functional transcriptional outcomes.