Anti-Infliximab (Remsima®)ADA Quantitative ELISA Kit

Assay Genie’s colorimetric, quantitative ELISA is designed to determine anti-infliximab antibodies against the Remsima® biosimilar in serum and plasma samples. It uses a sandwich assay format, with sample antibodies captured by biosimilar-coated microtiter wells and a peroxidase-labelled conjugate; reaction colour after TMB substrate addition is directly proportional to antibody concentration. The kit provides standards, controls, assay buffer, confirmation reagent, peroxidase conjugate, TMB substrate and stop solutions, wash buffer, adhesive film, and semi-log graph paper.

Required sample volume is “10 uL”, and the manufacturer lists total assay time as “140 min”. It reports a detection limit of “31 (ng/mL)” and spike recovery of “85-115%”. Optical density is measured at “450/650 nm”, with measurement completed within “30 min” after stop solution is added. This kit is for research use only.