Anti-Bevacizumab (Avastin®)ADA Qualitative ELISA Kit

This colorimetric ELISA from Assay Genie is intended for research use only. The Anti-Bevacizumab ADA Qualitative ELISA Kit qualitatively determines antibodies to bevacizumab in serum and plasma samples, with free drug listed as the application. It uses a double-antigen sandwich format: after sample incubation and washing, a peroxidase-labelled bevacizumab conjugate is added, and TMB chromogenic substrate detects the resulting bound enzymatic activity. Reaction colour intensity is proportional to the concentration of anti-bevacizumab antibodies in the sample.

The stated required volume is “10 μl” and total time is “140 min”. The reported detection limit is “30 ng/mL”, spike recovery is “85-115%”, and shelf life is “1 year”. Kit components include ready-to-use reactive control, ready-to-use negative control, assay buffer, peroxidase conjugate, TMB substrate solution, TMB stop solution, wash buffer concentrate, microtiter plate and adhesive foil.