Anti-Avelumab (Bavencio®)ADA Qualitative ELISA Kit

From Assay Genie, this qualitative ELISA kit is intended for the qualitative determination of antibodies to Avelumab (Bavencio®) in serum and plasma, and is for professional use only. It is a high-quality antibody-screening ELISA configured as a sandwich assay, in which anti-avelumab antibodies in patient serum or plasma samples are captured by the drug avelumab (Bavencio®) coated on the wall of microtiter wells.

After washing away unbound components from samples, a peroxidase-labelled specific conjugate is added to each well and incubated. The bound enzymatic activity is then detected colorimetrically by addition of TMB chromogen-substrate, and the reaction is terminated with an acidic stop solution. The intensity of the reaction colour is directly proportional to the concentration of anti-avelumab antibodies in the sample.

Avelumab (Bavencio®), also known as MSB0010718C, is a fully human anti-PD-L1 IgG1 lambda monoclonal antibody with a molecular weight of approximately 147 kDa. It is a programmed death-ligand 1 (PD-L1) blocking antibody used for the treatment of adults and pediatric patients greater than 12 years with metastatic Merkel Cell Carcinoma (MCC). By inhibiting PD-L1 interactions, avelumab is thought to enable the activation of T-cells and the adaptive immune system. The procedure requires a sample volume of 10 μL with a total assay time of 140 minutes, and the kit has a stated shelf life of 1 year.