Anti-Adalimumab (Humira®)Free Drug/ADA Dual ELISA Kit

This colorimetric ELISA from Assay Genie is a semi-quantitative screening kit for anti-adalimumab antibodies in serum and plasma. It enables simultaneous measurement of free and total anti-drug antibodies on the same plate and can also measure the presence of free drug in patient samples. The listed detection limit is 250 (ng/mL), the required sample volume is 10 μl, and the total assay time is 95 min.

For total-antibody testing, acid dissociation separates adalimumab-specific antibody–adalimumab immunocomplexes before the antibodies are captured on adalimumab-coated wells. For free-antibody screening, the dissociation and neutralisation steps are not required. The kit is for research use only.