Androgen Receptor (Phospho-Tyr363)Colorimetric Cell-Based ELISA Kit

Assay Genie supplies this Cell-Based ELISA Kit for the qualitative detection of phosphorylated Androgen Receptor (Phospho-Tyr363) directly in cultured cells. The lysate-free, high-throughput workflow uses an indirect ELISA format in which target-specific primary antibodies capture Androgen Receptor (Phospho-Tyr363) and HRP-conjugated secondary antibodies catalyze a colorimetric reaction measured at 450 nm. A monoclonal anti-GAPDH antibody is included to serve as an internal positive control, and a Crystal Violet whole-cell staining method is provided to normalise absorbance values to cell density, allowing plating differences to be adjusted.

The kit is configured with a dynamic range of > 5000 Cells and shows reactivity with Human, Mouse, and Rat samples, and is suitable for adherent cell preparations. It allows researchers to measure relative levels of phosphorylated Androgen Receptor and to screen the effects of treatments, inhibitors such as siRNA or chemical compounds, and activators on Androgen Receptor phosphorylation. Associated identifiers listed for the target include Gene ID: 367, UniProt ID: P10275, and Gene Symbol: AR, with the assay classified as a Phospho sub-type directed against the Androgen Receptor.