AMPKalpha (Phospho-Thr172)Colorimetric Cell-Based ELISA Kit

Manufactured by Assay Genie, this cell-based ELISA kit is designed for the colorimetric detection of AMPKalpha phosphorylated at Threonine 172 directly in cultured cells, using an indirect ELISA format with HRP-conjugated secondary antibodies and measurement at 450 nm. The assay is lysate-free and can be used to measure the relative levels of phosphorylated AMPKalpha in response to treatments, inhibitors, or activators, and is suited to high-throughput workflows. Reactivity is reported for human, mouse, and rat samples, with a stated dynamic range of > 5000 cells.

An anti-GAPDH monoclonal antibody is included as an internal positive control for normalizing target absorbance values, and a Crystal Violet whole-cell staining step can be used to determine cell density so that absorbance readings can be corrected for plating differences. The kit is a phospho-specific cell-based ELISA intended for research into AMPK signaling, a pathway implicated in cellular energy homeostasis, glucose uptake, lipid metabolism, and protein synthesis, with applications in studying metabolic and signaling responses in relevant disease models.