AML1 Transcription Factor Activity Assay

The Assay Genie AML1 Transcription Factor Activity Assay is a DNA-binding ELISA kit that quantifies the active DNA-binding form of AML1 (also known as RUNX1), a runt-family transcription factor essential for definitive hematopoiesis and frequently studied in acute myeloid leukemia research.

Nuclear and cell lysates are loaded into the provided microplate wells, where active AML1 protein binds to immobilized consensus DNA sequences; bound complexes are then detected using a transcription factor-specific antibody with an absorbance-based readout.

The kit is validated for reactivity with human, mouse, and rat AML1/RUNX1, supporting cross-species comparative studies in hematopoietic cell lines and primary cultures. Researchers use this in vitro assay to monitor changes in AML1 transcriptional activity following gene knockdown, pharmacological treatment, or differentiation stimuli, making it useful for studies of leukemogenesis, stem cell self-renewal, and transcription factor dysregulation.

Each kit provides reagents for 96 individual reactions, and components should be stored refrigerated upon arrival to preserve antibody and oligonucleotide activity.