AFB1 (Aflatoxin B1) ELISA Kit

The AFB1 (Aflatoxin B1) ELISA Kit from Assay Genie is a competitive immunoassay intended for quantitative detection of aflatoxin B1 in food commodities. Aflatoxin B1 is a potent hepatocarcinogen produced by Aspergillus flavus and A. parasiticus molds that frequently contaminate maize, peanuts, tree nuts, and cereal grains during cultivation and storage.

This assay is validated for a diverse set of food matrices including grain, wheat bran, peanut, biscuits, soy sauce, and vinegar, supporting food manufacturers, grain processors, and quality control laboratories working to meet international mycotoxin regulatory limits. The kit uses a competitive ELISA format in which free AFB1 in the sample competes with coated antigen for antibody binding sites, with a colorimetric readout measured on a standard microplate reader. The antibody provides preferential recognition of AFB1 with reduced cross-reactivity toward the related aflatoxins AFB2, AFG1, and AFG2.

Each kit provides reagents sufficient for 96 determinations, supporting routine batch testing of food samples. Intended for research use only, the assay facilitates mycotoxin surveillance and compliance screening in food safety laboratories.