Adrenergic Receptor B2 (Phospho-Ser355+Ser356)Colorimetric Cell-Based ELISA Kit

Assay Genie's Adrenergic Receptor B2 (Phospho-Ser355+Ser356) Colorimetric Cell-Based ELISA Kit is a cell-based, lysate-free assay designed for qualitative detection of phosphorylated Adrenergic Receptor B2 in cultured cells. The kit uses an indirect ELISA format in which target-specific primary antibodies capture the phosphorylated receptor, and HRP-conjugated secondary antibodies catalyze a colorimetric reaction measured at 450 nm.

Reactivity has been characterized for Human, Mouse and Rat samples, with a stated dynamic range of > 5000 Cells. The workflow is suited to adherent cells and supports high-throughput screening of treatments, inhibitors (such as siRNA or chemicals) or activators that may modulate receptor phosphorylation. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalizing target absorbance, while a Crystal Violet whole-cell staining step is provided to account for variation in cell density across wells.

Because detection is qualitative, results are interpreted by normalizing target absorbance to either the GAPDH internal control or to Crystal Violet-determined cell number. The kit targets ADRB2 (UniProt ID P07550).