ADD1/2 Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA Kit provides a convenient, lysate-free, high-throughput format for detecting ADD1/2 protein expression directly in cultured cells. The assay uses a colorimetric detection method read at 450 nm and is supplied in a 96-Well Microplate format, with reactivity against human, mouse, and rat samples.

The kit works on an indirect ELISA principle: ADD1/2-specific primary antibodies capture the target, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colour reaction upon substrate addition. A monoclonal anti-GAPDH antibody is included as an internal positive control, and Crystal Violet whole-cell staining can be used to determine cell density for normalisation of absorbance values. The dynamic range is greater than 5000 cells, making the kit suitable for measuring relative ADD1/2 levels and for screening the effects of treatments, inhibitors such as siRNA or chemicals, or activators.

ADD1 (alpha-adducin) and ADD2 (beta-adducin) are cytoskeletal proteins that act as heterodimers and promote assembly of the spectrin-actin network, with binding to calmodulin. According to the supplied database entries, ADD1 has Gene ID 118 and UniProt ID P35611, with a molecular weight of 73,333 Da. The kit is suitable for adherent cells, serum, plasma, and lysate samples, and is stored at 4 °C with a stated stability of 6 months.