ADA2L Transcription Factor Activity Assay

ADA2L (also known as TADA2A) operates as a transcriptional coactivator within the ATAC and SAGA/STAGA chromatin-modifying complexes, contributing to histone acetylation and the regulation of gene expression. The Assay Genie Transcription Factor Activity Assay quantifies the DNA-binding activity of nuclear ADA2L using an immobilized oligonucleotide approach, providing a functional readout rather than a simple protein concentration value.

The kit is validated for reactivity with human, mouse, and rat samples, enabling cross-species comparison in cell-based and animal studies. Nuclear and cell lysates are prepared using standard extraction protocols, and a typical sample volume of 10 to 20 uL is added per well of the microplate. Detection relies on an enzymatic absorbance-based reaction measured with a standard plate reader.

Reagents should be stored refrigerated; aliquot working solutions and limit freeze-thaw cycles to preserve reagent activity. Protect light-sensitive components from exposure. The kit is supplied for research use only and supports investigations in transcriptional regulation, chromatin biology, and studies of coactivator function in normal and disease states.