ABL1 (Phospho-Tyr245)Fluorometric Cell-Based ELISA Kit

From Assay Genie, this fluorometric cell-based ELISA kit enables qualitative detection of phosphorylated ABL1 at tyrosine 245 directly in cultured cells, with no lysate preparation required. ABL1 is a non-receptor tyrosine-protein kinase implicated in a range of cellular processes linked to cell growth and survival, including cytoskeleton remodelling, cell adhesion, and apoptosis. The indirect assay uses ABL1 (Phospho-Tyr245)-specific primary antibodies paired with Dye 1- and Dye 2-conjugated secondary antibodies, allowing fluorescent readout at Ex/Em wavelengths of 651/667 and 495/521.

Validated for human, mouse and rat cells, the kit supports high-throughput screening of treatments, inhibitors (such as siRNA or chemical compounds) and activators to study their impact on ABL1 phosphorylation. Normalisation is supported by an anti-GAPDH monoclonal antibody, which acts as an internal positive control, and an antibody against non-phosphorylated ABL1, which allows RFU values to be normalised against total ABL1 levels. Two primary antibody preparations — anti-phospho-target and anti-total target — are supplied to measure both phosphorylated and total ABL1.

The kit has a dynamic range of > 5000 Cells, is stored at 4°C with a stated 6 Months stability, and is configured as a cell-based phospho ELISA. Associated database references include Gene ID: 25, UniProt ID: P00519, OMIM #: 189980/608232, and Unigene #: Hs.431048.