8-epi-PGF2A/8-Epi-Prostaglandin F2 Alpha ELISA Kit

From Assay Genie, this competitive ELISA kit is designed for the precise measurement of 8-epi-PGF2α (8-epi-Prostaglandin F2 Alpha) in biological samples. The assay uses an antigen-coated, colorimetric competitive format with universal reactivity and accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

8-epi-PGF2α is recognised as a biomarker of oxidative stress and lipid peroxidation, with roles in inflammatory processes and various pathological conditions. Monitoring its levels can offer insights into oxidative damage, inflammation, and related disease pathways.

The kit provides a sensitivity of 9.375 pg/mL and a working range of 15.625-1000 pg/mL. Reported linearity (n = 10 per matrix) at 1:2, 1:4, and 1:8 dilutions is 84-100%, 82-101%, and 88-98% for serum; 93-103%, 89-99%, and 84-92% for EDTA plasma; and 89-98%, 85-103%, and 88-98% for heparin plasma. Recovery ranges and averages are 88-101% (91%) for serum, 88-100% (94%) for EDTA plasma, and 85-104% (94%) for heparin plasma. Store sealed at 2-8°C and do not cryopreserve.