60S Ribosomal Protein L10 Colorimetric Cell-Based ELISA Kit

Assay Genie supplies this cell-based ELISA kit for the qualitative determination of 60S Ribosomal Protein L10 in cultured cells. The assay is described as a convenient, lysate-free, high-throughput format using an indirect ELISA approach: target-specific primary antibodies capture 60S Ribosomal Protein L10, and HRP-conjugated secondary antibodies then catalyze a colorimetric reaction upon substrate addition, read at 450 nm. The kit is listed as reactive with human, mouse, and rat samples and uses a 96-well microplate format.

To support interpretation, a monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance values, and a Crystal Violet whole-cell staining step is provided for normalizing absorbance to cell density. Multiple normalization methods are recommended given the qualitative nature of the cell-based ELISA. The stated dynamic range is > 5000 cells, and the kit is intended to be stored at 4 °C with a stability of 6 months. The kit can be used to measure the relative amounts of 60S Ribosomal Protein L10 in cultured cells, including adherent cells, and to screen the effects of treatments, inhibitors such as siRNA or chemicals, or activators on the target.

The target is encoded by the RPL10 gene (UniProt ID P27635, Gene ID 6134), is a component of the large 60S ribosomal subunit, and plays a role in protein synthesis, with a stated molecular weight of 24,604 Da. The kit is categorized under signal transduction research, reflecting interest in the dysregulation of this protein in diseases such as cancer and its use as a biomarker for studying disease mechanisms.