5-HIAA ELISA Kit

Designed to accurately detect 5-Hydroxyindoleacetic acid (5-HIAA) levels in biological samples, this Competitive ELISA Kit from Assay Genie offers a highly sensitive and specific assay for researchers studying serotonin metabolism and related conditions. 5-HIAA is an important metabolite of serotonin, and abnormal levels may indicate conditions such as carcinoid tumors, serotonin-producing tumors, and certain psychiatric disorders. The kit supports a wide range of research applications across oncology, neurology, and psychiatry, providing reliable and reproducible results with an easy-to-use format and fast assay time.

The assay uses a competitive ELISA format coated with antigen and a colorimetric detection method, with a reported sensitivity of 0.938 ng/mL and a measuring range of 1.563–100 ng/mL. Reactivity is universal, and the kit accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Storage is at 2–8°C (sealed), and cryopreservation is not recommended. The procedure includes reagent and plate preparation, sample incubation with biotin-labeled antibody, washing, TMB substrate color development in the dark, stop reaction, and absorbance reading at 450 nm. Reported linearity at 1:2, 1:4, and 1:8 dilutions is 90–105% for serum, 82–101% for EDTA plasma, and 81–100% for heparin plasma, with recovery averages of 96% (serum), 97% (EDTA plasma), and 98% (heparin plasma).

Recognised synonyms for the target analyte include 5-HIAA and 5-Hydroxyindoleacetic Acid. Sample preparation guidance covers serum, plasma, cell culture supernatant, cell lysate, and tissue homogenate, with matrix-specific centrifugation conditions.