5-HETE (5-Hydroxyeicosatetraenoic Acid) ELISA Kit (UNFI0108)

Arachidonic acid metabolism through the 5-lipoxygenase pathway generates 5-hydroxyeicosatetraenoic acid (5-HETE), a bioactive eicosanoid linked to leukotriene synthesis, inflammatory cascades, and vascular signaling. Assay Genie's competitive ELISA kit enables quantification of 5-HETE in laboratory specimens for research applications including asthma studies, cardiovascular biomarker work, and lipid mediator profiling. The format supports parallel processing of blood-based specimens alongside standard curve replicates, with absorbance-based readout on a standard microplate reader. Reagents are provided as a complete kit and should be refrigerated upon receipt; protect components from light to preserve antibody activity. Each reaction is performed in vitro under the supplied protocol, intended strictly for research use only and not for clinical diagnosis. Buffer, conjugate, and detection antibody components support reproducible measurement across experimental batches.