2-Methoxy Estradiol ELISA Kit

From Assay Genie, this competitive ELISA kit is designed for the quantitative measurement of 2-Methoxy Estradiol, a natural metabolite of estradiol noted for its anti-angiogenic and antitumor properties. The assay is coated with antigen and is positioned for research applications including cancer research, biomarker discovery, and studies of angiogenesis, tumor growth, and related physiological and pathological processes.

The kit is suitable for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with reactivity described as Universal. Detection is colorimetric, and the kit should be stored sealed at 2-8°C without cryopreservation. The procedure uses a biotin-labeled antibody binding step followed by TMB substrate development, with absorbance measured at 450 nm using a microplate reader.

Reported performance includes a sensitivity of 18.75 ng/mL and a detection range of 31.25-2000 ng/mL. Linearity across 1:2, 1:4, and 1:8 dilutions is given for serum (86-102%, 90-105%, 89-103%), EDTA plasma (90-100%, 90-98%, 82-100%), and heparin plasma (83-98%, 82-98%, 81-98%). Recovery averages 97% in serum, 96% in EDTA plasma, and 94% in heparin plasma.