Virtual Hu Pulmonary Organoid Course

This virtual training program from STEMCELL Technologies delivers a comprehensive, protocol-driven introduction to establishing and maintaining human pulmonary organoid cultures. The live-streamed curriculum covers airway epithelial cell isolation, expansion in serum-free media systems such as PneumaCult, and three-dimensional lung organoid generation within basement membrane extract domes. Across multiple half-day sessions, participants follow lecture modules, demonstration videos, and step-by-step protocol walkthroughs covering growth factor supplementation, differentiation toward alveolar and airway lineages, and passaging strategies. Troubleshooting segments address common culture challenges including dome collapse, contamination, and inconsistent differentiation, while downstream modules discuss in vitro disease modeling and drug screening applications. Practical guidance includes incubation parameters such as 37°C with 5% CO2, centrifugation at 200 x g for cell pellet recovery, and reseeding densities around 50,000 cells per well in a 24-well plate format. The course is delivered as a research-use-only training resource for academic and translational researchers modeling lung biology, cystic fibrosis, COPD, and other respiratory conditions. Enrollees receive digital reference materials, including reagent lists and printable protocol PDFs, and gain access to interactive Q&A sessions with field experts. This curriculum supports reproducible pulmonary organoid workflows in independent laboratories entering the organoid biology space.