UCP SYBR Green PCR Kit (500)

From QIAGEN, this ultra-clean production (UCP) Master Mix kit is designed for real-time PCR and 2-step RT-PCR using SYBR Green I fluorescent dye, providing highly sensitive and rapid real-time quantification of DNA and cDNA targets in genomic DNA analysis and 2-step RT-PCR workflows (e.g., when paired with the QuantiNova Reverse Transcription Kit).

The 2x UCP SYBR Green PCR Master Mix contains UCP DNA Polymerase — a hot-start enzyme based on a modified recombinant 94 kDa DNA polymerase originally isolated from Thermus aquaticus — kept inactive at ambient temperature by an antibody and a novel Guard molecule and activated by a 2 min 95°C incubation step. The Master Mix also contains the UCP SYBR Green PCR Buffer (Tris, NH4+, K+, Mg2+, and additives) and a dNTP mix of dATP, dCTP, dGTP, and dTTP of ultrapure quality. A built-in visual pipetting control uses an inert blue dye in the Master Mix and an inert yellow dye in the UCP Yellow Template Dilution Buffer; combining the two turns the solution green. The kit is engineered for high inhibitor resistance against substances commonly found in microbiome sample types, with dedicated ultra-clean production processes minimising DNA background.

The kit is shipped on dry ice and should be stored at −30 to −15°C protected from light; the Master Mix, Template Dilution Buffer, and ROX Reference Dye may alternatively be stored at 2–8°C for up to 6 months. An optional UCP ROX Reference Dye is supplied in a separate tube for use on Applied Biosystems instruments requiring ROX passive reference dye normalisation. The UCP SYBR Green PCR Kit has been optimised for use with all currently available real-time cyclers. Each lot is tested against predetermined specifications under QIAGEN's ISO-certified Quality Management System. The product is intended for molecular biology applications and is not intended for the diagnosis, prevention, or treatment of disease.