Type-it HRM PCR Kit (2000)

This HRM (High Resolution Melt) PCR kit from QIAGEN is functionally validated for reliable detection of genetic differences. It is designed for applications including detection of SNPs, mutations and mutation scanning, making it suitable for genotyping and mutation discovery projects. The kit supports screening of large sample sets for SNPs, small insertions/deletions and sequence variants.

The reaction type is PCR amplification, performed with hot-start activation and including a Mastermix. The enzyme activity includes 5'->3' exonuclease activity, and the kit is supplied without ROX. Sequence-specific probes are not necessary, since EvaGreen dye for detection is included in the Mastermix. The intended sample/target type is genomic DNA, and the kit can be used for both real-time and endpoint analysis.

The HRM-tuned master mix and dye deliver reproducible melt curves for sensitive variant detection, with the design supporting minimal assay re-optimization across instruments and templates.