TeSR-E8-3D kit

An animal component-free, fed-batch suspension culture system from STEMCELL Technologies supports three-dimensional scale-up of human ES and iPS cells as uniform aggregates. Built on the TeSR-E8 formulation, this low-protein platform strips the workflow back to only the components essential for pluripotent cell expansion, supporting translational pipelines, cell therapy manufacturing, and regenerative medicine research.

The complete kit contains a 480 mL bottle of Seed Basal Medium, a 20 mL Seed Supplement for culture initiation, and a 20 mL Feed Supplement for daily nutrient replenishment. Supplementing with 10 µM Y-27632 at inoculation promotes single-cell survival and consistent aggregate formation, while the fed-batch strategy eliminates weekend medium exchanges. Validated suspension vessels include 100 mL and 250 mL Pfeiffer CELLSPIN spinner flasks, 300 mL polycarbonate round bottles, Applikon miniBio 500 bioreactors, and AggreWell microwell plates for size-controlled spheroid generation.

Recommended seeding densities range from 0.5 to 1 x 10^6 cells/mL, supporting expansion to roughly 10^9 undifferentiated hPSCs within two to three weeks. Pluripotency markers OCT4, NANOG, and SSEA-4 remain expressed across multiple passages with EDTA-based single-cell passaging and demonstrated karyotype stability. This human-specific formulation is compatible with cells previously maintained in mTeSR1 or TeSR-E8 on Corning Matrigel hESC-qualified matrix or Vitronectin XF substrates, bridging routine 2D maintenance with stirred-tank bioreactor scale-up for directed differentiation into the three germ layers, disease modeling, and high-throughput drug screening.

For storage, refrigerate the Seed Basal Medium protected from light and keep the Feed Supplement frozen at -20 °C; thaw the Feed Supplement overnight under refrigeration or at 15 to 25 °C before use. Provided as a research-use-only kit, the platform also enables bioprocess development and high-content screening workflows.