StemSpan-AOF

This animal origin-free (AOF) medium from STEMCELL Technologies is developed for the in vitro culture and expansion of human hematopoietic cells. Manufactured and tested following relevant cGMPs under a certified quality management system, it contains only recombinant proteins and synthetic components, with no serum or other human- or animal-derived components. Supplied in a single 500 mL format, it does not require the addition of serum or purified serum derivatives.

Using appropriate cytokines, the medium may be used to expand CD34+ cells isolated from human cord blood, mobilized peripheral blood, or bone marrow samples, or to expand and differentiate lineage-committed progenitor cells to generate populations of myeloid or megakaryocyte progenitor cells. It is described as a highly enriched basal medium, requiring only the addition of desired cytokines and growth factors needed to stimulate the cells of interest.

The product has traceability to the secondary level of manufacturing, meaning it is not manufactured using any raw material ingredients derived from an animal (including human) tissue, cell, or body fluid. The manufacturer has received a letter of acknowledgement from the FDA of its Master File. Storage: 2 - 8°C. Do not freeze. Stable until the expiry date on label.