STEMdiff Hematopoietic Supplement A

A defined, serum-free additive from STEMCELL Technologies, this hematopoietic specification supplement drives the differentiation of human pluripotent stem cells toward the blood lineage when combined with STEMdiff Hematopoietic Basal Medium and Supplement B. The formulation supports both monolayer and embryoid body workflows, providing cytokines and small-molecule cues that promote mesoderm induction and emergence of CD34+ hematopoietic progenitor cells over a 12-day protocol. Researchers typically pair the supplement with hPSC lines maintained under feeder-free conditions, refreshing the medium every two to three days to support robust hematopoietic commitment. The component arrives as a frozen vial intended for single-use thaw-and-aliquot handling; store at -20°C and protect from light, with shelf-life stability preserved by avoiding repeated freeze-thaw cycles. Applied in research use only cell culture workflows, the product enables downstream colony-forming assays, flow cytometry characterization of CD34/CD45 populations, and scale-up of progenitor yields for hematopoietic disease modeling and regenerative medicine studies.