STEMdiff Endothelial Expan Culture Kit

This xeno-free culture kit from STEMCELL Technologies is designed for the expansion of human pluripotent stem cell (hPSC)-derived endothelial cells. It supports efficient expansion while maintaining endothelial marker expression, and is suited to research areas including angiogenic cell research, disease modeling, and endothelial cell biology. After 7 days of mesoderm and endothelial induction, the resulting cells can be expanded for 5–6 passages to generate large numbers of highly pure, mature endothelial cells.

The kit contains xeno-free STEMdiff™ Endothelial Expansion Basal Medium (120 mL), STEMdiff™ Endothelial Expansion 5X Supplement (30 mL), and Animal Component-Free Cell Attachment Substrate (1 mL). The expanded cells typically reach ≥99% CD144+CD31+ by passage 2 and maintain this phenotype until passage 5. The same components are also included in the STEMdiff™ Endothelial Differentiation Kit.

The kit is compatible with hPSCs maintained in mTeSR™1, mTeSR™ Plus, or TeSR™-E8™ on Corning® Matrigel®. For passaging, an Animal Component-Free Cell Dissociation Kit is required, and Heparin Solution is required for preparation of the complete STEMdiff™ Endothelial Expansion Medium. This product is intended for research use only. A California Proposition 65 warning notes that the product can expose users to Nickel Compounds, which are known to the State of California to cause cancer and birth defects or other reproductive harm.