STEMdiff Cardio Freezing Medium

Cardiomyocyte cryopreservation reagent engineered for human pluripotent stem cell-derived cardiac cultures. This xeno-free freezing medium maintains contractile function and viability of differentiated cardiomyocytes through controlled-rate freezing workflows, supporting cardiac disease modeling, drug screening, and cardiovascular toxicology studies. Researchers use it to bank hPSC-derived cardiomyocytes generated through companion differentiation kits, enabling flexible scheduling of downstream assays without immediate plating pressure. The formulation supports post-thaw recovery with intact sarcomeric structure and spontaneous beating activity, and each 10 mL vial is intended for single-use aliquoting.

Store the freezing medium at -20 degrees C prior to first use. Once thawed, working aliquots remain stable at 4 degrees C for short-term handling, protected from light, and should not be subjected to repeated freeze-thaw cycles to preserve cryoprotectant efficacy. Supplied by STEMCELL Technologies as a workflow-integrated component of the cardiac differentiation and cardiomyocyte maintenance portfolio, in a format suited to research use only cell culture laboratories standardizing their cardiac cryopreservation protocols.