STEMdiff APEL2 Medium, 500 mL

STEMCELL Technologies STEMdiff APEL2 Medium is a defined, animal component-free differentiation medium formulated for the directed differentiation of human pluripotent stem cells (hPSCs), including embryonic stem cells and induced pluripotent stem cells. Based on the published APEL2 (Albumin, Polyvinylalcohol, Essential Lipids 2) formulation, this medium supports robust lineage commitment toward mesodermal derivatives such as hematopoietic progenitors, endothelial cells, and cardiomyocytes.

Researchers commonly use STEMdiff APEL2 Medium in embryoid body and monolayer-based protocols, supplementing with cytokines such as BMP4, VEGF, FGF2, and SCF to drive specific hematopoietic and endothelial outcomes. The 500 mL bottle provides sufficient volume for multiple differentiation experiments, with each lot performance-tested to support batch-to-batch consistency in mesoderm induction and hematoendothelial colony formation. The medium should be stored frozen at -20 °C, thawed at 4 °C prior to use, and is intended for research use only in cell culture workflows involving defined, xeno-free conditions.