SolisFAST® Lyo-Ready qPCR Kit with UNG

This qPCR kit from Solis BioDyne is an optimized solution compatible with lyophilization, enabling fast and sensitive DNA amplification in probe-based qPCR assays. It combines a glycerol-free qPCR mix with a lyophilization excipient mix, and a blend of the two is suitable for reliable lyophilization into lyo-cakes and lyo-beads. The critical temperature (Tc) of the kit is -30.8 °C and the glass transition temperature (Tg) of the lyophilized product is 68.3 °C.

The 5x SolisFAST® Lyo-compatible qPCR Mix with UNG is a 5x concentrated, glycerol-free master mix that supports real-time detection and quantitation of up to five DNA targets simultaneously. It contains the SolisFAST® DNA polymerase, an in silico-designed, inhibitor-tolerant analogue of Taq DNA polymerase with oligo-based hot-start and approximately 2-4 times faster extension rates than wild-type Taq. The mix uses dUTPs in place of dTTPs and includes Salini UNG® Uracil-N-Glycosylase to prevent carry-over contamination from previous amplifications. The MgCl2 concentration is 16.5 mM (1x mix – 3.3 mM). The 4x SolisFAST® Lyo Excipient Mix is a 4x concentrated blend of lyophilization additives that protect reagents during freeze-drying and stabilize the lyophilizates. The kit is compatible with qPCR cyclers that do not require an internal reference dye (such as ROX) for normalization of fluorescent signal.

Routine storage is at -20 °C until the Expiry Date; the kit is also stable at 4 °C (2-8 °C) for 5 months and at room temperature (25 °C) for 2 weeks, with freeze-thaw stability through 30 cycles. Reaction setup is performed at room temperature. The product is manufactured by Solis BioDyne in compliance with the ISO 9001 and ISO 13485 Quality Management Systems and is supplied for research use only.